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Ultrasensitive chemiluminescence determination method for pyrroloquinoline quinone in human plasma: a useful tool to investigate biological roles of pyrroloquinoline quinone

October. 3, 2017

Pyrroloquinoline quinone (PQQ) is considered as a potential vitamin found in many foods and it is used as a blood glucose level sensor. Since 2008, Mitsubishi Gas Chemical Co., Ltd. has commercialized PQQ disodium salt as a new food ingredient. Recently, much attention has been paid to the usefulness of PQQ in the field of medicine, biology and chemistry such as improvement of cognitive function, life-extension capability and its unique crystal structure.
The collaborative research group of Prof. Naotaka Kuroda in Graduate School of Biomedical Sciences, Nagasaki University and Dr. Kazuto Ikemoto in Mitsubishi Gas Chemical Co., Ltd. developed an ultrasensitive and selective chemiluminescence determination method for PQQ in human plasma. This method is based on the redox cycle of the reaction between PQQ and dithiothreitol, which generates reactive oxygen species that can be detected using luminol as a chemiluminescent probe. This method allows sensitive detection of PQQ at ppt (10-12) levels, and is successfully implemented to determine PQQ concentration in the plasma of healthy individuals after administration of PQQ supplements. Hence, the developed method is suitable for the determination of plasma concentration of PQQ to study the pharmacokinetics of this compound. In addition, the method can be utilized to elucidate in detail the distribution of PQQ by its application to other biological samples.
Furthermore, application of the developed method to study biological fate of other beneficial food ingredients is expected to contribute to find new potential drug or nutrient.



Paper Tittle:
Ultrasensitive determination of pyrroloquinoline quinone in human plasma by HPLC with chemiluminescence detection using the redox cycle of quinone.

Journal: Journal of Pharmaceutical and Biomedical Analysis http://www.sciencedirect.com/science/article/pii/S0731708517310105